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Table 1 Table 1

From: Estrogen protects against the synergistic toxicity by HIV proteins, methamphetamine and cocaine

Antigen Primary Dilution Antibody Secondary Specificity &
  antibodies/   Source antibodies/antisera Subcellular distribution
  antisera    (dilutions)  
Estrogen Mouse 1:10 Chemicon Goat anti-mouse IgG Neurons and astrocytes /
receptor IgG1   (MAB428) (H+L)conjugated to Cy3 Punctate nuclear and
(ER) monoclonal    (1:250) perinuclear distribution
Dopamine Rabbit 1:1000 Chemicon • Goat anti-rabbit IgG Neuronal processes and cell
(DA) IgG   (AB122S) DTAF (1:500) or body
  polyclonal    • Goat anti-rabbit IgG Cytoplasmic distribution
     Cy3 (1:250)  
Dopamine Rat 1:100 Chemicon Goat anti-rat IgG Neuronal processes and cell
transporter Ig type   (MAB369) conjugated to biotin- body; astrocytes
(DAT) monoclonal    avidin-Cy2 (1:250) Cell membrane
Dopamine Rabbit 1:100 Chemicon Goat anti-rabbit IgG Neurons and astrocytes;
D1A polyclonal   (AB1784P) DTAF (1:500) punctate distribution
receptor      
Dopamine Rabbit 1:500 Chemicon Goat anti rabbit IgG Neuronal processes and cell
D2 polyclonal   (AB1558) DTAF (1:500) bodies: perinuclear
receptor      distribution, astrocytes,
      microglia
Co-localization
Combined     • Goat anti-rabbit IgG Colocalized in neurons
• DA + as above as above as above DTAF + and/or astrocytes
• ER     • Goat anti-mouse  
     Cy3  
Combined     • Goat anti-rabbit IgG Colocalized in
• DA + as above as above as above Cy3 subpopulations of neurons
• DAT     • Goat anti-rat IgG and/or astrocytes
     biotin-avidin Cy2  
Combined as above as above as above • Goat anti-rabbit IgG Colocalized in
• D1A +     DTAF (1:500) subpopulations of neurons
• ER     • Goat anti-mouse and/or astrocytes
     Cy3 (1:250)  
Combined as above as above as above • Goat anti-rabbit IgG Colocalized in
• D2 +     DTAF (1:500) subpopulations of neurons
• ER     • Goat anti-mouse and/or astrocytes and
     Cy3 (1:250) microglia