Table 1

antisera

(dilutions)

Estrogen

Mouse

1:10

Chemicon

Goat anti-mouse IgG

Neurons and astrocytes /

receptor

IgG¹

(MAB428)

(H+L)conjugated to Cy3

Punctate nuclear and

(ER)

monoclonal

(1:250)

perinuclear distribution

Dopamine

Rabbit

1:1000

Chemicon

• Goat anti-rabbit IgG

Neuronal processes and cell

(DA)

IgG

(AB122S)

DTAF (1:500) or

body

polyclonal

• Goat anti-rabbit IgG

Cytoplasmic distribution

Cy3 (1:250)

Dopamine

Rat

1:100

Chemicon

Goat anti-rat IgG

Neuronal processes and cell

transporter

Ig type

(MAB369)

conjugated to biotin-

body; astrocytes

(DAT)

monoclonal

avidin-Cy2 (1:250)

Cell membrane

Dopamine

Rabbit

1:100

Chemicon

Goat anti-rabbit IgG

Neurons and astrocytes;

D1A

polyclonal

(AB1784P)

DTAF (1:500)

punctate distribution

receptor

Dopamine

Rabbit

1:500

Chemicon

Goat anti rabbit IgG

Neuronal processes and cell

D2

polyclonal

(AB1558)

DTAF (1:500)

bodies: perinuclear

receptor

distribution, astrocytes,

microglia

Co-localization

Combined

• Goat anti-rabbit IgG

Colocalized in neurons

• DA +

as above

as above

as above

DTAF +

and/or astrocytes

• ER

• Goat anti-mouse

Cy3

Combined

• Goat anti-rabbit IgG

Colocalized in

• DA +

as above

as above

as above

Cy3

subpopulations of neurons

• DAT

• Goat anti-rat IgG

and/or astrocytes

biotin-avidin Cy2

Combined

as above

as above

as above

• Goat anti-rabbit IgG

Colocalized in

• D1A +

DTAF (1:500)

subpopulations of neurons

• ER

• Goat anti-mouse

and/or astrocytes

Cy3 (1:250)

Combined

as above

as above

as above

• Goat anti-rabbit IgG

Colocalized in

• D2 +

DTAF (1:500)

subpopulations of neurons

• ER

• Goat anti-mouse

and/or astrocytes and

Cy3 (1:250)

microglia