Figure 7

BMP-5 immunoreactivity is detected in co-cultures of SCG neurons and glia. Phase contrast (A,C,E) and fluorescence (B,D,F) micrographs of SCG cultures immunostained for BMP-5 after 10 days in vitro. Both glial cells and neurons express significant BMP-5 immuno-reactivity, and in neurons, the processes as well as the soma are brightly stained (A,B). Preincubation of BMP-5 Ab with blocking peptide prior to reaction with SCG cultures (C,D) reduces the intensity of immunostaining to levels comparable to the background fluorescence observed in SCG cultures reacted only with secondary Ab (E,F).