Immunocytochemical staining of terminally differentiated cells. NPCs from group B were differentiated using the neuronal induction medium and were stained with FluoroPan neuronal marker for neurons and astrocytes (GFAP). Nuclei have been counterstained with SYTOX Blue. (A) Phase contrast image of differentiated cells. (B) FluoroPan neuronal FITC. (C) Nucleus staining with SYTOX Blue. (D) Overlay image. (E) Astrocytes stained with GFAP and counterstained with secondary antibody Cy3. Images were taken at 400× magnification using a Pascal 5 confocal microscope (Carl Zeiss, Germany).