PYK2 inhibiter abrogates the phosphorylation of β-catenin and rescues endothelial permeability. (A) Tyrosine phosphorylation of β-catenin demonstrated after PYK2 inhibition by applying chemical inhibiter PF-431396. PYK2 inhibition significantly abrogated the tyrosine phosphorylation of β-catenin even in HIV-1 Tat C exposed hBMVECs. Cells were treated with PF-431396 followed by treatment of HIV-1 Tat C protein and treated cells were harvested after 12 hours for western blot analysis. (B) Densitometry analysis showing the significantly diminished tyrosine phosphorylation of β-catenin in PF-431396 treated cells. All the experiments have been repeated as three independent biological sets and results are shown as mean ± S.E. (*p value ≤0.05). (C) The graph bars are showing consequences of inhibiting PYK2 kinase activity on endothelial permeability. PYK2 inhibiter provide significant rescuing of endothelial permeability as compared to HIV-1 Tat C treated human BMVECs (**p value ≤0.005). Results are shown as mean ± S.E of three biological independent experiments.