ROS scavenger (DPI) mediated rescue of SHP2 expression and downregulation in phosphorylation of β-catenin. (A) Western blot analysis for SHP2 expression level after DPI treatment and DPI treatment followed by HIV-1 Tat C treatment. DPI treatment shows significant potential for checking the HIV-1 Tat C mediated downregulation of SHP2 expression. (B) The graph bars are showing average change in SHP2 expression normalized by β-tubulin. Image densitometry analysis has been done by ImageJ software. (C) Western blot analysis showing effect of ROS inhibition on tyrosine phosphorylation of β-catenin. (D) The graph bars are representative of 3 independent experiments showing average change in tyrosine phosphorylation of β-catenin in DPI treated hBMVECs. P-value ≤0.05 has been considered as significant and shown as *between DPI + Tat C versus HIV-1 Tat C treated hBMVECs. (E) Change in TEER (Ώ/cm2) values after DPI treatment. The graph bars showing average change in endothelial permeability after scavenging ROS by DPI. All the experiments have been performed three times independently and data shown as mean ± S.E. P value ≤0.0005 shown as ***asterisk to indicate the level of significance between Tat C treated versus DPI + Tat C treated hBMVECs.