Effect of previous infection with S. aureus in body weight and clinical score. C57BL/6 mice were infected with S. aureus TSST-1+ and TOX-. Three days later, the animals were subjected to EAE induction, and their body weight and clinical scores were evaluated daily. The profile of the animals’ weight during 30 days (a), clinical score (b) and maximum clinical score (c). Data are presented as the mean ± SE of 9-12 mice and are representative of two independent experiments, p < 0.05. The statistical analysis (ANOVA or ANOVA on Ranks with Dunn’s method) indicates significant differences between EAE and both infected groups. These differences began on day 15 and remained significant until day 30. No differences were observed between the two infected groups.