Figure 4

Histograms of Log2 Fold Change for analysed genes, following single neurotrophin and equimolar mixture treatments. (Upper panel) Average |Log2 Fold Change| (absolute value across time points) in the two groups of genes, with the treatments with NGF (proNGF/NGF 0/10 ng/ml), proNGF (proNGF/NGF 20/0 ng/ml) and the equimolar mixture of proNGF/NGF 20/10 ng/ml. (Lower panel) Average standard deviation (across time points) of |Log2 Fold Change| in the two groups of genes, with the treatments with NGF (proNGF/NGF 0/10 ng/ml), proNGF (proNGF/NGF 20/0 ng/ml) and the equimolar mixture of proNGF/NGF 20/10 ng/ml. The mixture shows a weaker response in Group I genes, as underlined also by a much smaller variability across time. (****) 1-tail T-test with unequal variance, p < 0.0001. (*) 1-tail T-test with unequal variance, p < 0.05. Error bars are s.e.m.