Figure 5
From: The punctate localization of rat Eag1 K+channels is conferred by the proximal post-CNBHD region
Characterization of rEag1-II, III, and IV chimeric channels. (A) Schematic representation of the construction of rEag1-II, rEag1-III, and rEag1-IV chimeras. (B) Representative K+ currents recorded from Xenopus oocytes over-expressing the indicated rEag1 constructs. (C) Membrane localization of the GFP-rEag1 chimeric channels in HEK293T cells. Scale bar, 10 μm. (D) Expression of the GFP-rEag1 chimeric channels in DIV12 hippocampal neurons. Scale bar, 25 μm. (E) Quantification of the number of GFP puncta per neuron for the GFP-rEag1 chimeric channels. Note the presence of rEag2-like GFP puncta density in rEag1-II only. (*: significantly different from GFP-rEag1; t-test, p < 0.05)(#: significantly different from GFP-rEag2; t-test, p < 0.05)