The alignment of amino acid sequences of human and mouse GAD25. GAD25-1 protein of 223 amino acids is produced by Isoforms 3 to 8. GAD25-2 protein of 226 amino acids is produce by Isoforms 9 and 10. The last 11 amino acids (marked in orange) of GAD25-1 and 44 amino acids (marked in yellow) of GAD25-2 are unique to these proteins. * (asterisk) indicates positions which have a single, fully conserved residue; : (colon) indicates conservation between amino acids of strongly similar properties; . (period) indicates conservation between amino acids of weakly similar properties.