Transient DOM exposure induces both BDNF and TrkB over-expression in OHSC. (A) Experimental design and timeline: 13 days-in-vitro (DIV) slices were treated with 2 μM DOM for 24 h (insult) and then changed to a DOM-free medium. Protein samples were collected at the indicated days post insult (DPI). The fluorescent marker of cellular damage propidium iodide (PI, 2 μM) was present in the medium during and after DOM treatment. (B, C) The effects of transient DOM exposure on BDNF and TrkB expression at different time-points post-insult were analyzed by immunoblot as described in Materials and Methods. The blots correspond to representative experiments and values are the means ± SEM of at least three experiments performed from different cultures (*P < 0.05 vs. C).