Figure 7
From: LOC689986, a unique gene showing specific expression in restricted areas of the rodent neocortex
Cellular localisation of the endogenous and recombinant LOC689986 protein. Human HeLa cells were transiently transfected with a vector encoding LOC689986 as a V5 tagged protein, or as a C- or N- terminally YFP tagged protein. After 24 hours the cells were subjected to V5- or YFP immunocytochemistry. The recombinant LOC689986 proteins are expressed in both the nucleus and the cytosol. The fluorescent micrographs depict nuclei (DAPI), expressed recombinant LOC689986 (LOC689986) and a merged image. Bars are indicated in the DAPI pictures.