Cellular distribution of Mcl1 in rat brain after middle cerebral artery occlusion (MCAO). (A) Double immunostaining showed the overwhelming majority of Mcl1 co-localized with NEUN-positive neurons. By contrast, only a few Mcl1 positive cells were labeled with GFAP-positive astrocytes (B) or OX42-positive microglia/macrophages (C). (D-E) Double staining indicated that Mcl1 positive cells did not co-localize with caspase-3 or TUNEL positive cells in the ischemic brain, respectively. Cont: control group. Scale bars = 50 μm.