Figure 7

Changes in Slc6a17 expression levels in hypothalamus in overnight starved and long term food restricted (45% of ad lib food intake), as well as in hippocampus and hypothalamus in a model for the monoamine system. (A-B) Three groups (n = 8 rats/group) were compared within a control group with free access to food (dark grey), a food deprived group (white), and a food restricted group (light grey). (A) In the restricted animals, the gene expression of Slc6a17 significantly (p = 0.0461) was up-regulated in hypothalamus. (B) The Slc6a15 gene was not significantly up- or down-regulated in any of the studied tissues. (C-D) Four groups (n = 8 rats/group) were compared within a control group given saline (dark gray), a group given 3 mg/kg fluoxetine (white), a group given 10 mg/kg fluoxetine (light grey) and a group given 25 mg/kg bupropion (black dots). (C) The Slc6a17 gene was significantly (p = 0.0009) up-regulated in hippocampus after low and high dose of fluoxetine application, while a non significant up-regulation was seen after bupropion treatment. Bupropion injection caused a significant up-regulation of Slc6a17 in hypothalamus (p = 0.0002) and in prefrontal cortex (p = 0.0005). (D) Significant (p = 0.0006) up-regulation of Slc6a15 was seen in hippocampus after injection of both doses of fluoxetine and bupropion and the Slc6a15 gene was significantly (p = 0.0002) up-regulated in prefrontal cortex after bupropion treatment. All data were normalized to the mean value for the control group. Significance levels were obtained from one-way ANOVA followed by Tukey’s post hoc test on a data set with p < 0.05 from the ANOVA. Multiple comparison test significant levels are indicated by asterisks (*, p < 0.05; **, p < 0.01; ***, p < 0.001).