Spatial and temporal expression patterns of LmigOR s. a, Tissue-specific expression of LmigORs in olfactory organs. M Ant, male antenna; F Ant, female antenna; Mou, mouthparts, Tarsi, locust tarsi; Wing, locust wings; Gut, locust gut. b, Dynamical expression of LmigORs in antenna during locust development. Egg, locust eggs; 1st–5th, locust antenna of nymphs from 1st to 5th instar; Adult, locust adult antenna. The locust actin gene was used as control for the integrity of the cDNA template. Amplification products were analysed on agarose gels and visualized by UV illumination after ethidium bromide staining. All tissues were dissected from gregarious locusts unless indicated.