Figure 7

Effect of 10 μM RA on the expression of functional voltage-sensitive. Ca²⁺ channels in M17 cells. M17 cells were treated with 1 mCi/ml ³H-Valine 24 hours prior to each experiment. M17 cells were either (A) undifferentiated cells stimulated for 4 minutes with KCL, or differentiated cells (B) stimulated for 4 minutes with KCl, (C) KCl + 10 μM NNC 55-0396/KCl, (D) KCl + 1 mM w conotoxin, GVIA, or (E) KCl + 300 nM w agatoxin IVA. Each of these KCl solutions contained 1 mCi/ml of ⁴⁵Ca²⁺. The ratio of ⁴⁵Ca²⁺/³H was then used to calculate the percentage difference of Ca²⁺ channel activity. % of control was calculated by dividing the experimental ratio of ⁴⁵Ca²⁺/³H by the ratio of ⁴⁵Ca²⁺/³H generated with 5.9 mM KCl alone. n=4 *p<0.05 when compared to 5.9 mM KCl. **p<0.01 when compared to 5.9 mM KCl.