Figure 2

RIC-3 increases whole cell and cell surface expression of α-Bgt binding sites. Spectral confocal microscopy images of α-Bgt binding assays using Alexa 648 α-Bgt (Fl-Bgt) and performed under nonpermeabilizing conditions (A, B, C, D) without RIC-3, or (E, F, G, H) with RIC-3 (1:1 to α7), and permeabilizing conditions (I, J, K, L) without RIC-3 and (M, N, O, P) with RIC-3 (1:1 to α7). Emission signals from α7-Cerulean, α7-Venus, Fl-Bgt binding sites and the merged α7-Venus / Fl-Bgt images are shown for each corresponding cell. RIC-3 increases surface and intracellular α-Bgt binding sites.