Effect of CHF5074 and DAPT treatments on neuronal cell-cycle functionality. A-F. Representative images of cyclin A immunoreactivity (red staining, B and E) in NeuN-positive cells (green staining, C and F) and of the resulting merge images (A, D) in Tg2576-mice treated with vehicle (A-C) or with CHF5074 375 ppm (D-F); # indicates the animal code. G. Quantification of cyclin-A positive cells versus total NeuN-positive cells in the different treatment groups. While approximately 50% of neurons express Cyclin A in untreated (“vehicle”) Tg2576 mice, the percentage of Cyclin A/NeuN-positive neurons is reduced to less than 20% in CHF5074-treated, but not DAPT-treated, animals. * p < 0.05 and ***p < 0.001 vs vehicle-treated Tg2576 mice; c p < 0.001 vs CHF5074 125 and 375 ppm-treated Tg2576 mice.