Developmental differences in the effect of mAChR activation on spontaneous neuronal activity recorded from cortical cultures via MEA. (A) The relationship between culture developmental stage (DIV) and firing type following mAChR activation by 10 μM OXO-M. Outer box bottom = 25th percentile, top = 75th percentile, error bars bottom = 5th percentile, top = 95th percentile, central line = median, central square = mean. B) Network spike profile (see Table 1) for immature (grey) and mature (black) cultures presented as normalised change to network spiking rate vs control following mAChR activation. t0 = time bin at which maximum firing rate was detected.