Figure 7

P^S129α-syn accumulates in fibrillar deposits in hA30Pα-syn mice. Representative electron micrographs illustrating a cell body (A) and a process sectioned transversally (B) or longitudinally (C) and immunostained with P^S129α-syn antibodies and secondary antibodies coupled to horseradish peroxidase from a representative symptomatic hA30Pα-syn mouse expressing parkin. (A’-B’) are higher magnifications of the framed regions in (A) and (B). (D) Nanogold staining of a P^S129α-syn deposit within a neuronal process. Arrowheads indicate fibrils with (red) or without (black) associated P^S129α-syn staining; N (nucleus), M (mitochondria). Similar results were obtained in hA30Pα-syn mice with two or no functional parkin alleles. Scale bars: 4 μm in (A) and (B), and 0.5 μm in (A’), (B’), (C) and (D).