Figure 3

The effect of Shh on motor neurons and motor neuron ciliation. Representative microscopic images show there were more SMI32+ cells (green) co-stained with a cilial marker ACIII (red) in Shh 250 ng/ml treated cell culture (B) than in control culture (A) from mSOD mice. In WT cells, both doses of Shh significantly increased the percentage of motor neurons in culture and significantly increased the percentage of ciliated motor neurons (C- note the different y axes). A similar situation occurred in the mSOD cells (C). * p<0.001, Shh (250 ng/ml and 500 ng/ml) vs control (0) for motor neuron percentage and ciliated motor neuron percentage in both WT and G93A SOD1 mice.