Figure 3

Impact of AdOx on the expression of Nanog and Oct3/4 in P19 cells. A. Nanog and Oct3/4 expression in P19 cells with AdOx (AdOx) or without (Control) as determined by Western blot. GAPDH is a loading control. Numbers indicated quantification of the Nanog or Oct3/4 western signal relative to GAPDH respectively. Digits at the bottom of the first & second row indicate the relative darkness of each band compared with the control lane set as 1.0. B. Impact of AdOx on the relative luciferase activity of the nanog and oct4 promoters. pGL3-nanog-luc (driven by the -289/+117 fragment of the nanog gene) and pGL-oct3/4-luc (driven by the -2143/+30 fragment of the oct4 gene) were individually co-transfected with pRL-TK into P19 cells treated with AdOx or without. The relative promoter activities showed that AdOx has a different effect on the two genes in P19 cells. Annotations are as described in Figure 1G.