Figure 3From: Decreased expression of extracellular matrix proteins and trophic factors in the amygdala complex of depressed mice after chronic immobilization stressThe expression of osteoglycin in the amygdala was analyzed using immunohistochemistry (IHC) in the three groups; CTL-MI, STR-HI, and STR-LI. The representative IHC result shows specific decreased expression of osteoglycin in the central (CeA), basolateral (BLA), and medial (MeA) nuclei of the amygdala of STR-HI mice (A). The fluorescence signal density of each nucleus was quantified using the Image J program (NIH), (B) and the strength of IHC was evaluated with one-way ANOVA and Tukey’s multiple-comparison test among groups. All tested nuclei showed significant differences in signal intensity among groups (F values of CeA, BLA, and MeA were 8.79, 14.70, and 13.55, respectively). As a result, only STR-HI mice showed significant signal decreases in each nucleus. Data are the means ± SEM (n = 3). a, P < 0.05 between CTL-MI and STR-HI mice; c, P < 0.05 between STR-HI and STR-LI mice. Scale bars are 200 μmBack to article page