GluR2 mRNA expression during normoxic and hypoxic aestivation. mRNA levels were evaluated in whole brain of FW (white1), 6dAE (black2), 6mAE (vertical striped3), 6dMUD (diagonal striped4) and 6mMUD (grey5) animals by qPCR. (a) The results are expressed as a proportion of the highest value after normalization with respect to β-actin levels and represent the means ± s.e.m. of three independent biological replicates. GluR2 in situ hybridization pattern was handled in the different brain nuclei of diencephalic (c) and extradiencephalic (d) areas of all aestivating conditions (expressed as % of FW). In particular, an evident increase of mRNA levels was detected in 6mAE (ii) with respect to 6mMUD (iii) lungfish at diencephalic level (b), of which a schematic representation was reported (i). Statistical analysis: ANOVA followed by Student’s t test for qPCR data and by Newman Keul’s test for in situ hybridization (ap < 0.05, bp < 0.01, cp < 0.001). Scale bar: 600 μm. For abbreviations check list.