Reduction in feeding does not affect organismal development.
50 embryos from the genotypes elav/w1118 (control), elav/THK, elav/THA and elav/THA;THK (increasing neuronal knockdown of DTH) and elav/UAS27.6 (increased DTH expression in the CNS), or elav/w1118 (control) and elav/TRHE;TRHA, were established in 16 replicate vials. A - C. Manipulation of neuronal TH levels. A. Pupal size . B. Time to pupariation. C. Reduction in number of pupae. **p < 0.01, ***p < 0.001, two way ANOVA followed by Bonferroni's post-tests. D, comparison of feeding rate between elav/w1118, elav/THA;THK, and elav/TRHE;TRHA. ***p < 0.001, one way ANOVA followed by Dunnett's post-test. E - F. Manipulation of neuronal TRH levels. E. Pupal size for the DTRH double transgenic knockdown line. F. Increase in number of pupae. ***p < 0.001, two way ANOVA followed by Bonferroni's post-test.