Figure 3From: Neurotrophic actions of dopamine on the development of a serotonergic feeding circuit in Drosophila melanogasterConstitutive reduction in neuronal DTH results in results in aberrant gut fiber architecture. Analysis of proventricular tissues from 3rd larval instars dissected and incubated with anti-5-HT. A. Neurite branching. B. Number of large varicosities (> 1 μm2) per 0.1 mm neurite length. C. Number of varicosities > 5 μm2 per 0.1 mm length. D. varicosity area. *p < 0.05, ***p < 0.001, one way ANOVA followed by Dunnett's multiple comparisons post-test. w1118/THA;THK, n = 66 fibers from 48 guts from 8 independent experiments; elav/THK, n = 40 fibers from 18 guts from 2 independent experiments; elav/THA, n = 37 fibers from 21 guts from 2 independent experiments; elav/THA;THK, n = 35 fibers from 18 guts from 3 independent experiments. Lines above the graph depict standard error of the mean. E - H. Visual depiction of 5-HT axonal fibers from w1118/THA;THK (E), elav/THK (F), elav/THA (G) and elav/THA;THK (H) guts. Scale bar = 30 μm.Back to article page