Neuronal knockdown of both TRH and TH during development of the circuit results in normal feeding. elavC155, pan-neuronal driver; DTRHGal4, drives expression in 5-HT neurons; DTHGal4, drives expression in DA neurons. A. TRHE, THK, weaker RNAi transgenes. B. TRHA, THA, stronger RNAi transgenes. n = 40 for behavioral analyses, from 4-6 independent experiments. ***p < 0.001, one way ANOVA followed by Tukey's multiple comparisons post-test. Lines above the graph depict standard error of the mean.