Table 2 The CUS procedure
Time | Course of the experiment |
|---|---|
The 1st day 9:00 | cage rocking (5 times per second) for 15 min |
The 2nd day 9:00 | swimming in cold (4°C) water for 5 min |
The 3rd day 9:00 | soiled cage (250 ml of tap water into the sawdust bedding) for 24 h |
The 4th day 9:00 | lights on overnight |
The 5th day 9:00 | cage tilting for 24 h |
The 6th day 9:00 | restraint for 12 h |
The 7th day 8:00 | food and water deprivation for 24 h |
The 8th day 8:00 | one-bottled-sucrose test sessions (last 1 h) |
9:00 | soiled cage for 24 h |
The 9th day 9:00 | electric stimulus (1.0 mA, each time for 1 second, 10 times per minute) for 5 min |
The 10th day 9:00 | elevated temperature (40°C) for 15 min |
The 11th day 9:00 | cage rocking for 15 min |
The 12th day 9:00 | lights on overnight |
The 13th day 9:00 | restraint for 12 h |
The 14th day 8:00 | food and water deprivation for 24 h |
The 15th day 8:00 | one-bottled-sucrose test sessions (last 1 h) |
9:00 | lights on overnight |
The 16th day 9:00 | swimming in cold water for 5 min |
The 17th day 9:00 | restraint for 12 h |
The 18th day 9:00 | electric stimulus for 5 min |
The 19th day 9:00 | cage rocking for 15 min |
The 20th day 9:00 | soiled cage for 24 h |
The 21th day 9:00 | cage tilting for 24 h (The CUS procedure finish) |
The 22th day 6:30 | weighed body weight and open field test |
8:00 | food and water deprivation for 24 h |
The 23th day 8:00 | one-bottled-sucrose test sessions (last 1 h) |