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Table 2 The CUS procedure

From: Pre-gestational stress reduces the ratio of 5-HIAA to 5-HT and the expression of 5-HT1A receptor and serotonin transporter in the brain of foetal rat

Time Course of the experiment
The 1st day 9:00 cage rocking (5 times per second) for 15 min
The 2nd day 9:00 swimming in cold (4°C) water for 5 min
The 3rd day 9:00 soiled cage (250 ml of tap water into the sawdust
bedding) for 24 h
The 4th day 9:00 lights on overnight
The 5th day 9:00 cage tilting for 24 h
The 6th day 9:00 restraint for 12 h
The 7th day 8:00 food and water deprivation for 24 h
The 8th day 8:00 one-bottled-sucrose test sessions (last 1 h)
9:00 soiled cage for 24 h
The 9th day 9:00 electric stimulus (1.0 mA, each time for 1 second,
10 times per minute) for 5 min
The 10th day 9:00 elevated temperature (40°C) for 15 min
The 11th day 9:00 cage rocking for 15 min
The 12th day 9:00 lights on overnight
The 13th day 9:00 restraint for 12 h
The 14th day 8:00 food and water deprivation for 24 h
The 15th day 8:00 one-bottled-sucrose test sessions (last 1 h)
9:00 lights on overnight
The 16th day 9:00 swimming in cold water for 5 min
The 17th day 9:00 restraint for 12 h
The 18th day 9:00 electric stimulus for 5 min
The 19th day 9:00 cage rocking for 15 min
The 20th day 9:00 soiled cage for 24 h
The 21th day 9:00 cage tilting for 24 h (The CUS procedure finish)
The 22th day 6:30 weighed body weight and open field test
8:00 food and water deprivation for 24 h
The 23th day 8:00 one-bottled-sucrose test sessions (last 1 h)
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