AFM image shows the forms of Aβ
after preparation. Aa-c: 100 nM Aβ after preparation at 0, 2 and 4 hrs. Red arrows indicate the small oligomers (Aa), small and large oligomers (Ab) and large oligomer and protofibriles (Ac). Ad-f: 1 nM Aβ at 0, 2 and 4 hrs after preparation. B: When dissolved with DMSO, monomer is the major form of Aβ after 2 hrs aggregation (Lane a). 9 Mer is the major form of Aβ, when dissolved with ACSF, after 2 hrs aggregation (Lane b).