Effect of bupivacaine or H
on WDR35 expression. (A) Neuro2a cells were treated with various concentrations of bupivacaine or H2O2 for 9 h. WDR35 mRNA expression was analyzed by qPCR and expressed relative to the expression of GAPDH mRNA. (B) WDR35 mRNA expression and (C) WDR35 protein expression were measured after treatment of Neuro2a cells with 2 mM bupivacaine or 0.5 mM H2O2 for the time indicated. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control (not treated with bupivacaine or H2O2, n = 6).