Figure 4

Assessment of the inflammatory properties of Wnt3a in microglia. Microglia (1 million cells per treatment) were treated with either Wnt3a (10 nM) or LPS (10 ng/ml) for 8 (A) or 24 hours (B) in SFM at 37°C. Control cultures were left in SFM for the corresponding time points. Cell culture supernatants were then collected and analysed for the presence of cytokines using a 10-plex immuno-fluorescent assay. Microglia (250000 cells per treatment) were treated with either Wnt3a (10 nM) or LPS (10 ng/ml) for 24 hours and then subjected to western blotting for iNOS and β-actin (C). Control microglia were cultured in SFM for 24 hours. *** p < 0.001.