Figure 1From: Development of synaptic connectivity onto interneurons in stratum radiatum in the CA1 region of the rat hippocampusMultiple connections between CA3 and CA1 pyramidal cells and GABAergic cells and CA1 pyramidal cells in young adult rats. (A1) A typical recording at -80 mV from a pyramidal cell, upper trace shows AMPA sEPSCs under control conditions and lower trace shows mEPSCs after wash-in of TTX. (A2) TTX reduces the AMPA EPSC amplitude by 5.9 ± 0.8 pA (P = 0.004, n = 8, two-tailed paired t-test). (A3) TTX reduces AMPA EPSC frequency by 1.5 ± 0.4 Hz (P < 0.001, n = 8, one-tailed paired t-test). (A4) The average multiplicity was 1.7 ± 0.1 (P < 0.001, n = 8, one-tailed one-sample t-test with test value = 1). (B1) A typical recording at 0 mV from a pyramidal cell, upper trace shows GABA sIPSCs under control conditions and lower trace shows mIPSCs after wash-in of TTX. (B2) TTX reduces the GABA IPSC amplitude by 8.4 ± 2.7 pA (P = 0.03, n = 6, two-tailed paired t-test). (B3) TTX reduces GABA IPSC frequency by 3.2 ± 0.8 Hz (P = 0.005, n = 6, one-tailed paired t-test). (B4) The average multiplicity was 2.4 ± 0.4 (P = 0.007, n = 6, one-tailed one-sample t-test with test value = 1). Black filled circles show mean ± SEM and green filled circles show data from individual experiments. Asterisks denote the level of significance as follows: *: P < 0.05, **: P < 0.01, ***: P < 0.001.Back to article page