Kalirin isoforms occur in many tissues. A. The major isoforms of Kalirin are diagramed. The qPCR primers used to quantify expression of different regions of the Kalirin gene are indicated and antibody specificities are indicated. DH, Dbl homology; PH, Pleckstrin Homology; GEF1, Guanine nucleotide exchange factor 1; SH3, Src homology domain; GEF2, Guanine nucleotide exchange factor 2; I, Immunoglobulin; F, Fibronectin III; Kin, Kinase domain; red lines, unique 5′- and 3′-untranslated regions. B. Quantitative PCR was used to determine expression of different regions of the Kalirin gene. Data are from individual preparations of RNA and cDNA from 4–8 mice for each tissue. The data were averaged across assays, typically 4 assays per tissue. C. PolyA-containing RNA was prepared from adult rat cortex and liver, and hybridized with 32P]-labeled probes for the GEF2 domain (1023 nt) or the region immediately 3′ to the Kal9 stop codon (530 nt) . Rps26 is ribosomal protein S26, used as a loading control.