Table 1 List of primary antibodies used for immunofluorescence and immunohistochemical analysis
Antibodies | Cell type | Origin | Company | Dilution | Antibody pre-treatment | Abbreviation |
|---|---|---|---|---|---|---|
anti-ASH1 (MASH1) | neurogenic precursors | rabbit polyclonal | Millipore | 1:20 | Citrate buffer | Mash1 |
anti-bromodeoxyuridine | proliferative cells | mouse monoclonal | Millipore | 1:50 | HCl | BrdU |
anti-carnosine | immature and mature OSNs | rabbit polyclonal | Millipore | 1:50 | TE buffer | carnosine |
anti-CD15 | glandular cells | mouse monoclonal | BD Biosciences | 1:10 | Citrate buffer | CD15 |
anti-glial fibrillary acidic protein | olfactory ensheathing glia | rabbit polyclonal | Dako | 1:250 | nil | GFAP |
anti-keratin | HBCs | rabbit polyclonal | Dako | 1:200 | nil | keratin |
anti-Ki-67, clone SP6 | proliferative cells | rabbit monoclonal | Thermo Scientific | 1:50 | nil | Ki67 |
anti-olfactory marker protein | mature OSNs | goat polyclonal | Santa Cruz Biotechnology | 1:800 | TE buffer | OMP |
anti-Pax6, C-terminus | neurogenic precursors | rabbit polyclonal | Millipore | 1:200 | Citrate buffer | Pax6 |
anti-phospho-Histone H3 [pSer10] | mitotic marker | rabbit polyclonal | Sigma | 1:200 | nil | pH3 |
anti-βIII Tubulin | immature and mature OSNs | mouse monoclonal | Promega | 1:800 | nil | βIIITubulin |