In vitro characterization of NPCs derived from microdissected periventricular regions throughout the brain. A: Schematic brain images showing the regions which were microdissected for NPC culture. B: Limiting dilution assay for primary neurospheres from different regions of control mice are shown (n=3). C: Limiting dilution assay for primary neurospheres from different regions of MPTP-treated mice are shown (n=3-6). Data are shown as mean±SEM, dotted lines represent 95% confidence interval (95%CI). The intercept of log (37% negative wells) gave the neurosphere-forming frequency. D-E: Representative neurospheres of NesGFP mice are depicted after one week of expansion of control (D) and MPTP-treated animals (E). Scale bar, 100 μm.