Figure 3From: High yield derivation of enriched glutamatergic neurons from suspension-cultured mouse ESCs for neurotoxicology researchRotary conditions increase NPC yield during differentiation. A. Bright-field images of ESCs differentiated under static (left) or rotary conditions (right; 45 rpm). Rotary conditions produced spherical aggregates without agglomeration. B. Average NPC yields for each cell line at DIV 0. Rotary culture of cell aggregates improves neural progenitor cell recovery approximately three-fold (P < .001, n=5).Back to article page