Fluoxetine prevents development of an early stress-related molecular signature in the rat infralimbic medial prefrontal cortex. Implications for depression?

Table 3 Primers used for validation of stress-responsive genes by RT-qPCR

Gene ID	Symbol	Sense (5^′-3^′)	Antisense (5^′-3^′)	Amplicon size (bp)
18S ribosomal RNA	Rn18s	CCCGAAGCGTTTACTTTGAA	CCCTCTTAATCATGGCCTCA	136
Glyceraldehyde-3-phosphate dehydrogenase	Gapdh	GAAGGGCTCATGACCACAGT	GGATGCAGGGATGATGTTCT	117
Actin, beta	Actb	CACACTGTGCCCATCTATGA	CCGATCGTGATGACCTGACC	272
Calcium/calmodulin-dependent protein kinase II alpha	Camk2a	GCCTGGACTTTCATCGATTC	GGTACTGAGTGATGCGGATGT	141
Glycogen synthase kinase 3 beta	Gsk3β	GCGAGACACACCTGCCCTCTTC	GTGGCCAGAGGTGGGTTACTTGAC	66
Mammalian target of rapamycin (serine/threonine kinase)	mTOR	TTGGATGTTCCAACCCAAGT	CAGGCCTTGGTTACCAGAAA	106
Neurotrophic tyrosine kinase, receptor, type 2	Ntrk2	TGGAGGGCGACCCACTCATCA	TCAGCTCGG TGGGCGGGTTA	123
Neurotrophic tyrosine kinase, receptor, type 3	Ntrk3	CATCCGCTGGATGCCACCTGAAA	AAGACACGGCCTTGGGTGATGCA	50
Phosphoinositide-3-kinase, catalytic, beta polypeptide	Pik3cb	CCTGCGACAGATGAGTGATG	CAATCCTCCGGTTGTCAAGT	134
V-raf murine sarcoma viral oncogene homolog B1	Braf	CATGGCGACGTGGCAGTGAAAATG	TGAGGTGTGGGTGCTGTCACATTC	50
Glyceraldehyde-3-phosphate dehydrogenase-3-prime	Gapdh-3 ^′	GGCTGGCATTGCTCTCAA	GAGGTCCACCACCCTGTTG	88
Glyceraldehyde-3-phosphate dehydrogenase-5-prime	Gapdh-5 ^′	GACAGCCGCATCTTCTTG	CACCGACCTTCACCATCTTG	63
Actin, beta-3-prime	Actb-3 ^′	CCTAGCACCATGAAGATCAAGA	GCCAGGATAGAGCCACCAATC	77
Actin, beta-5-prime	Actb-5 ^′	ACCCAGATCATGTTTGAGACCTT	CAGAGGCATACAGGGACAAC	79

ISSN: 1471-2202