Figure 5
Effect of Diva/BclB and NDPKB/Nm23H2 on the cell cycle and cellular proliferation of cells. (A) The number of cells in S phase are visualised by BrdU staining (green) in the nucleus. Diva/BclB had the highest proportion of stained nuclei, followed by control and NDPKB/Nm23H2 had the lowest proportion. (B) An average of 500 cells per group was counted, and quantitative analysis of the data showed that overexpression of NDPKB/Nm23H2 decreased the percentage of S-phase cells, while Diva/BclB increased cellular proliferation. (C) Growth curves of the control and transfected cells were plotted using MTS assay. Diva/BclB cells had a less inhibited growth curve, while NDPKB/Nm23H2 cells had a slower growth curve, when compared to control cells. (D-G) Flow cytometry data was collected after serum starvation for 24 hours (0hr), and at indicated times after serum addition. Graphical analysis using Summit software showed that overexpression of Diva/BclB resulted in a decrease in apoptotic cells and faster cell cycle progression. In contrast, overexpression of NDPKB/Nm23H2 resulted in slower cell cycle progression. (Bar=20μm, *p<0.05, **p<0.01).