Expression pattern of miR-125b and Nestin in NS/PCs during differentiation. Expression pattern of miR-125b in NS/PCs during differentiation quantified by real-time PCR using specific Taqman primers. U6 was used as the internal control. In the meantime, the expression level of Nestin was quantified by real-time PCR during NS/PCs differentiation. GAPDH was used as the internal control. Data represent mean±SD of three different experiments.