HuPrP106-126 induces [3 H]-AA release after 24 hours in primary cortical neurons. (A) Primary cortical neurons were incubated with 1 μCi ml-1 [3 H]-AA for 24 hours. Cells were washed twice with media and treated with 1 μM PMA + 5 μM A23187, 40 μM HuPrP106-126 or HuPrP106-126scram for 0, 15 or 30 minutes. Levels of [3 H]-AA were measured in cell lysates and supernatant to calculate a% [3 H]-AA release. (B) Experiment was repeated with cells treated for 24 hours. Data expressed as mean ± S.D. of duplicate samples from three experiments (n = 6). **P < 0.01, ***P < 0.001.