Figure 3

CREB phosphorylation in sustentacular cells is dependent on ACIII-mediated olfactory signaling. (A) Cryosections (12 μm) of OMP-GFP mice treated for 2 h with a mixture of 100 odorants or pre-treated with an inhibitor of adenylyl cyclase III (SQ22536). (B) High magnification image (2X) showing the inhibition of CREB phosphorylation in the sustentacular cell layer (arrowhead) and in the OSN cell soma (arrow), by an inhibitor of ACIII. Scale bar 10 μm. (C) Mean ± SD fluorescence intensity across the nuclei was quantified as before. Sustentacular cells showed a significant decrease from (6.47 ± 0.90, n = 79) after 2 h odorant treatment to (0.95 ± 0.24, n = 72) with SQ22536 pre-treatment; OSN nuclei decreased from 4.36 ± 3.47 (n = 70) after 2 h to 0.80 ± 1.39 (n = 81) with SQ22536 pre-treatment; immature cell nuclei did not change (2.90 ± 0.89 after 2 h (n = 80), 2.95 ± 0.86 (n = 74) with SQ22536 pre-treatment); p** < 0.01, p*** < 0.001.