Figure 1From: Purinergic receptor antagonists inhibit odorant-mediated CREB phosphorylation in sustentacular cells of mouse olfactory epitheliumOdorants induce MAPK and CREB phosphorylation in the olfactory epithelium. (A) Cryosections (12 μm) of mice expressing GFP in every mature OSN (OMP-GFP) were subjected to immunohistochemistry using an antibody specific for phosphorylated p42/44 MAPK in the olfactory epithelium. After 2 hours of odorant exposure a strong anti-phospho-p42/44 MAPK immunoreactivity was observed in the cytoplasm of OSNs. (B) Staining for phospho-CREB (Ser-133). Phospho-CREB was observed in the sustentacular cell layer, in the nuclei of OSNs, and in immature OSNs not expressing OMP, after exposure to a mixture of 100 different odorants for 2 h. Control panel with secondary antibody only or after using a specific blocking peptide shows very low background labeling intensity in the nuclei of the different cell types. (C) WB analysis of control cell lysates (SK-N-MC cells prepared with IBMX and forskolin treatment) and olfactory epithelium samples with CREB and phospho-CREB (Ser-133) specific antibodies showing labeling of the proteins (bands marked by stars), which is abolished when the specific blocking peptide was pre-incubated with the antibody.Back to article page