Figure 7

GSPE decreases glutamate- and 0.1 mM [Mg²⁺] _o -induced NO formation. Cells were preincubated with 20 μM DAF-2 DA for 60 min. NO formation was shown as a percentage of the initial intensity of DAF-2T. Treatment with glutamate (100 μM) or 0.1 mM [Mg²⁺]_o for 1 h significantly increased DAF-2T fluorescence. A: Pretreatment with GSPE (6 μg/ml) decreased the 100 μM glutamate-induced NO formation 65 min after 100 μM glutamate treatment (control, n = 9; 100 μM glutamate, n = 13; 100 μM glutamate + 6 μg/ml GSPE, n = 12). *P < 0.05 relative to 100 μM glutamate (unpaired Student's t-test). B: Pretreatment with GSPE (6 μg/ml) decreased the 0.1 mM [Mg²⁺]_o-induced NO formation 15 min after 0.1 mM [Mg²⁺]_o treatment (control, n = 10; 0.1 mM [Mg²⁺]_o, n = 14; 0.1 mM [Mg²⁺]_o + 6 μg/ml GSPE, n = 9). Data are expressed as the mean ± SEM. *P < 0.05 relative to 0.1 mM [Mg²⁺]_o (unpaired Student's t-test).