Colocalization of transplanted BMMCs with DCX and densitometry of DCX expression at various surviving times. Panels A to E show that transplanted BMMC pre-labeled by PKH26 (red) and DAPI (blue) co-localize with doublecortin (DCX, green) in the cerebral cortex of a recipient mouse (14 days surviving). (F) demonstrates DCX immunoreactivity visualized with the DAB method localizing around the border of the infarct area. (G) shows semi-quantitative measurements of DCX positive cells in MCA occluded mice at different time points. ANOVA table on the right summarizes statistical analysis data on the time course effect (longitudinal factor) in the 4 animal groups as well as treatment effect (transversal factor) at the 4 time points.