Figure 4

Alterations of NADPH-d reactivity in the HN and DMN at 3, 7, 14 and 28 days after PNCI and EGCG treatment. Quantitative analysis shows that PNCI causes a remarkably-increased population of NADPH-d(+) neurons at different time intervals examined. PNCI-induced NADPH-d(+) neurons reach their population peak at 7 days in the HN but continue to increase their population in the DMN until the end of the examination. Note a significant decrease of NADPH-d(+) neurons in the groups receiving EGCG treated animals when compared with those of non-treated (saline-treated) ones at 7 and 14 days in the HN. At the same time intervals in the DMN, the groups receiving high dosages of EGCG (25 and 50 mg/kg) show a marked reduction of NADPH-d(+) neuron population when compared with those receiving lower dose of EGCG (10 mg/kg) or saline. *, p < 0.05 for the comparison between EGCG-treated and saline-treated groups at the same survival time point.