Skip to main content

Table 1 Metabolite/5-HT and metabolite/DA ratios in the cortex, hypothalamus, caudate-putamen, midbrain and brainstem/pons of WT and Mecp2-/y.

From: Biogenic amines and their metabolites are differentially affected in the Mecp2-deficient mouse brain

Brain Area Group 5HIAA/5HT ratio (HVA + DOPAC)/DA ratio
Cortex P35 Wt 1.68 (+/- 0.14) 1.71 (+/- 0.42)
  P35 Ko 1.88 (+/- 0.29) 1.33 (+/- 0.43)
  P55 Wt 1.50 (+/- 0.17) 0.75 (+/- 0.10)
  P55 Ko 1.47 (+/- 0.29) 0.77 (+/- 0.12)
Hypothalamus P35 Wt 1.92 (+/- 0.26) 0.85 (+/- 0.06)
  P35 Ko 2.61 (+/- 0.37) * 0.94 (+/- 0.12)
  P55 Wt 1.37 (+/- 0.12) 0.73 (+/- 0.15)
  P55 Ko 1.54 (+/- 0.22) 0.62 (+/- 0.09)
CaudatePutamen P35 Wt 1.91 (+/- 0.11) 0.47 (+/- 0.03)
  P35 Ko 2.64 (+/- 0.22) ** 0.62 (+/- 0.05)
  P55 Wt 1.06 (+/- 0.13) 0.30 (+/- 0.02)
  P55 Ko 1.02 (+/- 0.08) 0.33 (+/- 0.02)
Midbrain P35 Wt 1.51 (+/- 0.23) 1.66 (+/- 0.14)
  P35 Ko 1.79 (+/- 0.27) 1.64 (+/- 0.09)
  P55 Wt 1.06 (+/- 0.13) 1.07 (+/- 0.13)
  P55 Ko 1.02 (+/- 0.08) 0.99 (+/- 0.04)
Brainstem + Pons P35 Wt 2.27 (+/- 0.39) 0.36 (+/- 0.03)
  P35 Ko 2.18 (+/- 0.31) 0.43 (+/- 0.09)
  P55 Wt 1.17 (+/- 0.06) 0.44 (+/- 0.06)
  P55 Ko 2.00 (+/- 0.51) * 0.39 (+/- 0.02)
  1. Shown are mean values +/- S.E.M. for the ratios of dopaminergic metabolites HVA + DOPAC/DA and serotonergic metabolite 5-HIAA/5-HT in the cortex (Cx), the hypothalamus (Hyp), the caudate-putamen (CPu), midbrain (Md) and brainstem + pons (BP) of Mecp2-/y and their WT littermates at P35 and P55. Dopamine turnover was unafected in the Cx, Hyp, CPu, Md and BP at both P35 and P55 in Mecp2-/y compared to their age-matched WT littermates. Serotonergic turnover was not modified in Cx and Md at any age investigated. However, Hyp and CPu dosage revealed a significant increase of 5-HIAA/5-HT ratio at P35 but not P55. In turn, values from the BP indicate a clear increase in the serotonergic turnover in Mecp2-/y when they exhibit the most severe phenotype (P55). (*p < 0.05, **p < 0.01).