Physiological properties of enkephalin-containing neurons in the spinal dorsal horn visualized by expression of green fluorescent protein in BAC transgenic mice

Table 1 Primers used for single-neuron reverse transcription-polymerase chain reaction (RT-PCR)

Gene	Name	Primer Sequences	Sequence Start	Product Length, bp	GenBank Accession Number
PPE	PPE-F	5'-CTACAGGCGCGTTCTTCTCT-3'	273	201	NM_001002927
	PPE-R	5'-AGCAGCAAACAGGATGA-3'	561
GAD67	GAD67-F	5'-CAAGTTCTGGCTGATGTGGA-3'	1616	231	NM_008077
	GAD67-R	5'-GCCACCCTGTGTAGCTTTTC-3'	1846
5HT3R	5HT3-F	5'-CGTCAAGTCTGGTTTCCTTACC-3'	1794	112	NM_013561
	5HT3-R	5'-GCATCTATGCAAGATGATTTGC -3	1905

PPE, preproenkephalin; GAD67, glutamate decarboxylase 67 gene; 5HT3R, 5-hydroxytryptamine (5-HT, serotonin) type 3 receptor gene. Two 5-HT₃ receptor subunits were cloned, namely subunit A (5-HT_3A) and subunit B (5-HT_3B). Although expression of 5-HT_3A subunits alone yields functional 5-HT₃ receptors, it has been suggested that 5-HT_3B subunits modify the physiological and pharmacological properties of 5-HT₃ receptors [35, 36]. In this study we attempted to detect the 5-HT_3A subunits. The primers were used for both the first and the second PCR amplification.

ISSN: 1471-2202