Figure 6

Abnormal expression of peroxiredoxin 6, tropomyosin, and lactate dehydrogenase in SMN-deficient ES cells. Control (C) and SMA (S) ES cells as described in 3A were processed for western blotting using antibodies against peroxiredoxin 6 (PRDX6), tropomyosin (TPM1/3), and lactate dehydrogenase (LDH), respectively. The same blot was stripped and re-probed with anti-ERK antibody for protein loading control (A). Signals of three independent experiments were quantified, normalized to ERK, and plotted as for 3B. Western blotting confirms that SMA ES cells show significantly higher levels of peroxiredoxin 6, tropomyosin, and lactate dehydrogenase expression (** p < 0.01 and * p < 0.05) (B). Undiff. = undifferentiated cells and Diff. = differentiated cells.