Figure 7

Normal lamellar spacing in Fa2h^-/-/ Cgt^-/- myelin. (A) Typical cross sections of spinal cords from Cgt^-/- and Fa2h^-/-/Cgt^-/-mice (two different magnifications are shown). Arrows indicate axons with thin or without myelin. Asterisks indicate demyelinated axons. Scale bar, 40 μm (upper panel) 10 μm (lower panel). Myelin thickness (B) and g-ratios (C) were determined in the spinal cord of three mice per genotype. Though there was a shift towards increased myelin thickness in Fa2h^-/-/Cgt^-/- compared to Cgt^-/- mice (Data shown are the mean SD [n = 3 animals per genotype]; **p < 0.01, Chi-Square test), the g-ratios of myelinated axons were similar in Cgt^-/- and Fa2h^-/-/Cgt^-/- mice. (D) Electron micrographs showing normal compact myelin in Fa2h^-/-/Cgt^-/- mice (Magnification: 16,000×). Scale bar, 50 nm.