Figure 5

Reduced levels of MBP and compact myelin in Fa2h^-/-/ Cgt^-/- and Cgt^-/- mice. (A) Western blot analysis of 4-week-old wild-type (lanes 1 and 2), Cgt^-/- (lanes 3 and 4), and Fa2h^-/-/Cgt^-/- mice (lanes 5 and 6) brain homogenates showed reduced MBP levels in Cgt^-/- and Fa2h^-/-/Cgt^-/- mice. Shown is one representative out of three independent experiments, all of which gave comparable results. (B) The dry weight of purified myelin per mg brain wet weight isolated from Cgt^-/- and Fa2h^-/-/Cgt^-/- mice (n = 3 per genotype) was significantly reduced compared to wild-type controls (*p < 0.05, t-test).