Effect of TRPM8 ASODN on expression of TRPM8 protein in ipsilateral L5 DRGs of CCI rats. Immunohistochemical staining (A) and western blot analysis (B) of TRPM8 protein in L5 DRGs from sham-operated rats treated with MMODN or ASODN and CCI rats treated with MMODN or ASODN. TRPV1 immunoreactivity (B) is used to examine the specificity of TRPM8 antisense experiment. Data are expressed as mean ± SEM. **P < 0.01 vs. CCI-MMODN rats; §§P < 0.01 vs. Sham-MMODN rats (one-way ANOVA followed by SNK test). Magnification of immunohistochemical images is 400 ×, and scale bars represent 25 μm. Arrows indicate TRPM8 positive neurons.